Ratio cluster plot. — ratio_cluster_plot • PICtR

Stacked bar plot of each cluster with the proportion of cells below/above the threshold determined with Otsu's method using the FSC.A/FSC.H ratio.

Usage

ratio_cluster_plot(
  obj,
  clusters = "seurat_clusters",
  ratio = "ratio_anno",
  assay = "FACS"
)

Arguments

obj: The Seurat object.
clusters: The string of the meta.data column with the clustering resolution to plot.
ratio: The meta.data column with the classification of cells (ratio_high/ratio_low) determined using the FSC.A/FSC.H ratio and the determined threshold using Otsu's method.
assay: The Seurat assay to use (default FACS).

Value

None